Comparison of <Emphasis Type="Italic">Mycobacterium tuberculosis</Emphasis> isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity,oligomeric state,pH tolerance and phylogenetic affiliation |
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Authors: | Sharmistha?Banerjee Ashok?Nandyala RaviPrasad?Podili Vishwa?Mohan?Katoch Email author" target="_blank">Seyed?E?HasnainEmail author |
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Institution: | (1) Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam,Hyderabad, 500076, India;(2) Central JALMA Institute for Leprosy, Tajganj,Agra, 282001, India;(3) Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Jakkur,Bangalore, 560012, India |
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Abstract: | Background
M.tb icd-1 and M.tb icd-2, have been identified in the Mycobacterium tuberculosis genome as probable isocitrate dehydrogenase (ICD) genes. Earlier we demonstrated that the two isoforms can elicit B cell
response in TB patients and significantly differentiate TB infected population from healthy, BCG-vaccinated controls. Even
though immunoassays suggest that these proteins are closely related in terms of antigenic determinants, we now show that M.tb icd-1 and M.tb icd-2 code for functional energy cycle enzymes and document the differences in their biochemical properties, oligomeric assembly
and phylogenetic affiliation. |
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Keywords: | |
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