| 酪氨酸酶电流传感器测定低浓度氰化物抑制剂的研究 |
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| 引用本文： | 胡效亚,冷宗周. 酪氨酸酶电流传感器测定低浓度氰化物抑制剂的研究[J]. 高等学校化学学报, 1995, 16(3): 359-362 |
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| 作者姓名： | 胡效亚 冷宗周 |
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| 作者单位： | 扬州大学师范学院化学化工系, 扬州 225002 |
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| 基金项目： | 江苏省教育委员会自然科学基金 |
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| 摘 要： | 用预活化聚酰胺膜固定酪氨酸酶,制得性能良好的酪氨酸酶生物传感器,以此测定CN-抑制剂的含量。在磷酸盐缓冲溶液中,以苯酚作底物,浓度范围为5×10-6～4×10-5mol/L,当苯酚浓度一定时,还原电流(-0.200V,vs,SCE)的下降值与CN-浓度的平方根成线性关系,检测范围为5×10-7～2×10-4mol/L,有良好的选择性和重现性。
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| 关 键 词： | 电极 生物传感器 酶 酪氨酸酶 氰化物 |
| 收稿时间： | 1994-04-09 |
Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide |
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| HU Xiao-Ya, LENG Zong-Zhou. Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide[J]. Chemical Research In Chinese Universities, 1995, 16(3): 359-362 |
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| Authors: | HU Xiao-Ya LENG Zong-Zhou |
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| Affiliation: | Department of Chemistry and Chemical Engineering, Teachers College, Yangzhou University, Yangzhou 225002 |
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| Abstract: | Cyandide inhibitor of tyrosinase was determied with biosensor of tyrosinase by using tyrosinase immobilized on a preactivated polyaminde membrane.Phenol with the range of concentration 5×10-6～4×10-5mol/Lwas chosen as substrate in phosphate buffer.The de-crease of substrate response have linear relation with square root of concentration of CN-with detection range of 5×10-7～2×10-4mol/L. The fine selectivity and reproducibility were obtained. |
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| Keywords: | Electrode Biosensor Enzyme Tyrosinase Cyanide |
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