On-chip free-flow magnetophoresis: Separation and detection of mixtures of magnetic particles in continuous flow |
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| Authors: | Nicole Pamme Jan CT Eijkel Andreas Manz |
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| Institution: | 1. Department of Chemistry, Imperial College London, South Kensington, London SW7 2AY, UK;2. Department of Chemistry, The University of Hull, Cottingham Road, Hull HU6 7RX, UK;3. BIOS/Lab-on-a-Chip Group, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;4. Institute for Analytical Sciences (ISAS), Bunsen-Kirchhoff Strasse 11, 44139 Dortmund, Germany |
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| Abstract: | The complete separation of mixtures of magnetic particles was achieved by on-chip free-flow magnetophoresis. In continuous flow, magnetic particles were deflected from the direction of laminar flow by a perpendicular magnetic field depending on their magnetic susceptibility and size and on the flow rate. 2.8 and 4.5 μm superparamagnetic particles with magnetic susceptibilities of 1.1×10−4 and 1.6×10−4 m3 kg−1, respectively, could be completely separated from each other reproducibly. The separated particles were detected by video observation and also by on-chip laser light scattering. Potential applications of this separation method include sorting of magnetic micro- and nanoparticles as well as magnetically labelled cells. |
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| Keywords: | 75 50 T |
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