Determination of gamma-globulin at nanogram levels by its enhancement effect on the resonance light scattering of functionalized HgS nanoparticles |
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Authors: | Wang Le-Yu Wang Lun Dong Ling Hu Yan-Li Xia Ting-Ting Chen Hong-Qi Li Ling Zhu Chang-Qing |
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Affiliation: | College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China |
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Abstract: | A novel assay of γ-globulin (γ-IgG) with a sensitivity at the nanogram level is proposed based on the measurement of enhanced resonance light-scattering (RLS) signals resulting from the interaction of functionalized nano-HgS with γ-globulin. At pH 5.03, the RLS signals of functionalized nano-HgS were greatly enhanced by γ-globulin in the region of 200-700 nm characterized by the peak around 362 nm. Linear relationship can be established between the enhanced RLS intensity and γ-globulin concentration in the range of 10-140 ng ml−1. The limit of detection is 2.71 ng ml−1. Based on this, a new direct quantitative determination method for γ-globulin in blood serum samples without separation of human serum albumin (HSA) is established. The contents of γ-IgG in blood serum samples were determined with recovery of 95.7-102.5% and R.S.D. of 1.6-2.4%. This method is proved to be very sensitive, rapid, simple and tolerance of most interfering substances. |
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Keywords: | Resonance light scattering γ-Globulin Nano-HgS |
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