Determination of urinary oxidative DNA damage marker 8-hydroxy-2'-deoxyguanosine and the association with cigarette smoking |
| |
Authors: | Yao Qing-Hong Mei Su-Rong Weng Qian-Feng Zhang Pu-Duen Yang Qing Wu Cai-Ying Xu Guo-Wang |
| |
Institution: | a National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, ZhongShan Road 161, Dalian 116011, PR China b College of Chemistry, Wuhan University, Wuhan 430072, PR China |
| |
Abstract: | 8-hydroxy-2′-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30 mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3 nM (signal-to-noise ratio S/N=3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers ( versus , P=0.0004; creatinine versus creatinine, P=0.028). |
| |
Keywords: | 8-hydroxy-2&prime -deoxyguanosine Urine Cigarette smoking Capillary electrophoresis End-column amperometric detection |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|