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Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenase |
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| Authors: | Harald Gröger Werner Hummel Claudia Rollmann Hendrik Hüsken Christine Wunderlich Karlheinz Drauz |
| Institution: | a Degussa AG, Project House Biotechnology, P.O. Box 1345, 63403 Hanau, Germany b Institut für Molekulare Enzymtechnologie der Heinrich-Heine-Universität, Forschungszentrum Jülich, Wilhelm-Johnen-Straβe, 52426 Jülich, Germany c Degussa AG, SU Process Technology, P.O. Box 1345, 63403 Hanau, Germany d Degussa AG, BU Fine Chemicals, FC-TRM, P.O. Box 1345, 63450 Hanau, Germany |
| Abstract: | The substrate range of a novel recombinant (S)-alcohol dehydrogenase from Rhodococcus erythropolis is described. In addition, an enzyme-compatible biphasic reaction medium for the asymmetric biocatalytic reduction of ketones with in situ-cofactor regeneration has been developed. Thus, reductions of poorly water soluble ketones in the presence of the alcohol dehydrogenase from R. erythropolis and a formate dehydrogenase from Candida boidinii can be carried out at higher substrate concentrations of 10-200 mM. The resulting (S)-alcohols were formed with moderate to good conversion rates, and with up to >99% ee. |
| Keywords: | Ketones In situ-cofactor regeneration (S)-Alcohol |
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