Preparation and evaluation of non-bonded hyperbranched polymer-coated columns for capillary electrophoresis |
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| Authors: | Shou Chong-Qi Zhou Chang-Li Zhao Chun-Bin Zhang Zhi-Liang Li Guan-Bin Chen Li-Ren |
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| Institution: | a Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China
b Department of Applied Chemistry, Jinan University, Jinan 250022, PR China |
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| Abstract: | A series of hyperbranched poly(amine-ester)s based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine were synthesized and coated on the inner surface of the fused-silica capillaries by physical adsorption. The most effective coating was the seventh generation hyperbranched poly(amine-ester) coating, which reduced the electroosmotic flow (EOF) greatly and suppressed protein adsorption effectively. The high separation efficiencies for basic proteins were obtained and the coating had a good stability. |
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| Keywords: | Capillary electrophoresis Protein Coated column Hyperbranched polymer |
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