Near-infrared fluorimetric determination of nucleic acids by shifting the ion-association equilibrium between tetracarboxy aluminum phthalocyanine and tetra-N-hexadecylpyridiniumyl porphyrin |
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Authors: | Zhuo Shu-Juan Zheng Hong Chen Jin-Long Li Dong-Hui Wu Yu-Qin Zhu Chang-Qing |
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Affiliation: | a College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China b The Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China c Cancer Research Center of School of Life Science, Xiamen University, Xiamen 361005, China |
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Abstract: | A new method was developed for determination of micro amounts of nucleic acids based on near-infrared (near-IR) fluorescence recovery, employing a two-reagent system which is composed of an anionic tetracarboxy aluminum phthalocyanine (AlC4Pc) and a cationic tetra-N-hexadecylpyridiniumyl porphyrin (TC16PyP). The fluorescence of the AlC4Pc, with the maximum emission wavelength at 701 nm, could be quenched by TC16PyP at its proper concentration, but recovered by adding nucleic acids. Under optimal conditions, the recovered fluorescence is proportional to the concentration of nucleic acids. The calibration graphs are linear over the range of 1-200 ng mL−1 for fish sperm DNA (FS DNA) and 2-400 ng mL−1 for calf thymus DNA (CT DNA). The corresponding detection limits are 0.59 ng mL−1 for FS DNA and 0.82 ng mL−1 for CT DNA, respectively. Four synthetic and three real nucleic acid samples were determined with satisfactory results. |
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Keywords: | Near-IR fluorescence Tetracarboxy aluminum phthalocyanine (AlC4Pc) Tetra-N-hexadecylpyridiniumyl porphyrin (TC16PyP) Nucleic acids |
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