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Analysis of tryptophan at nmoll(-1) level based on the fluorescence enhancement of terbium-gadolinium-tryptophan-sodium dodecyl benzene sulfonate system |
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| Authors: | Liu Shufang Yang Jinghe Wu Xia Su Benyu Sun Changxia Wang Feng |
| Institution: | a Key Laboratory of Colloid and Interface Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Ministry of Education, Jinan 250100, PR China b Shandong Supervision and Inspection Institute for Product Quality, Jinan 250100, PR China c Department of Chemistry, Zaozhuang Teacher’s College, Zaozhuang 277160, PR China |
| Abstract: | It is found that Tb3+ can react with tryptophan (Trp) and sodium dodecyl benzene sulfonate (SDBS), and emits the intrinsic fluoresence of Tb3+. The fluorescence intensity can be enhanced by La3+, Gd3+, Lu3+, Sc3+ and Y3+, among which Gd3+ has the greatest enhancement. This is a new co-luminescence system. The studies indicate that in the Tb-Gd-Trp-SDBS system, there is both Tb-Trp-SDBS and Gd-Trp-SDBS complexes, and they aggregate together and form a large congeries. The fluorescence enhancement of the Tb-Gd-Trp-SDBS system is considered to originate from intramolecular and intermolecular energy transfers, and the energy-insulating sheath effect of Gd-Trp-SDBS complex.Under the optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of Trp in the range from 4×10−8 to 4×10−5 mol l−1. The detection limit is 10−9 mol l−1. The proposed method is one of the most sensitive fluoremetries of Trp. |
| Keywords: | Energy transfers Fluorescence enhancement Tryptophan Determination Rare earth ion |
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