Enzymatic-chemical isolation of lipids,proteins, and gossypol from cotton seeds and meal. I

An enzymatic-chemical method of isolating the complex of lipids, gossypol pigments, and water-soluble proteins from cotton seeds and meal has been developed which permits: a) the achievement of the maximum yield of all types of lipids without their degradation; b) a rise in the qualitative and food value of the oil and protein products isolated from the seeds, with an increase in the solubility of the latter; c) the extraction from cotton seeds of more than 75% and from cottonseed meal of 42% of the free gossypol. The method, in combination with Folch's procedure, is suitable for the quantiative estimation of the lipids, strongly bound to the protein of the nucleus.