Oxidative Decarboxylation of Short‐Chain Fatty Acids to 1‐Alkenes |
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Authors: | Dr Alexander Dennig Miriam Kuhn Sebastian Tassoti Anja Thiessenhusen Dr Stefan Gilch Dr Thomas Bülter Dr Thomas Haas Dr Mélanie Hall Prof Dr Kurt Faber |
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Institution: | 1. Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria);2. Creavis, Evonik Industries, Bau 1420, Paul Baumann Strasse 1, 45772 Marl (Germany) |
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Abstract: | The enzymatic oxidative decarboxylation of linear short‐chain fatty acids (C4:0–C9:0) employing the P450 monooxygenase OleT, O2 as the oxidant, and NAD(P)H as the electron donor gave the corresponding terminal C3 to C8 alkenes with product titers of up to 0.93 g L?1 and TTNs of >2000. Key to this process was the construction of an efficient electron‐transfer chain employing putidaredoxin CamAB in combination with NAD(P)H recycling at the expense of glucose, formate, or phosphite. This system allows for the biocatalytic production of industrially important 1‐alkenes, such as propene and 1‐octene, from renewable resources for the first time. |
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Keywords: | 1‐alkenes biocatalysis fatty acids OleT oxidative decarboxylation |
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