| 9-芴甲氧羰酰氯柱前衍生-高效毛细管电泳法测定人血浆中牛磺酸 |
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| 引用本文： | 薛勇,薛丰,孙成均. 9-芴甲氧羰酰氯柱前衍生-高效毛细管电泳法测定人血浆中牛磺酸[J]. 分析试验室, 2011, 30(6) |
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| 作者姓名： | 薛勇 薛丰 孙成均 |
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| 作者单位： | 四川大学华西公共卫生学院,成都,610041 |
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| 摘 要： | 建立了区带毛细管电泳法快速测定人血浆中牛磺酸的方法.血浆样品经乙腈沉淀蛋白并离心后,上清液中牛磺酸与9-芴甲氧羰酰氯在室温及pH 9.5条件下避光反应20 min,生成具有紫外吸收的衍生产物,以40 mmol/L的乙酸钠(pH 4.6)为运行缓冲溶液,熔融石英毛细管为分离柱;分离电压22kV;紫外检测.实验结果表明:在优化的实验条件下,样品检测仅需9 min,牛磺酸质量浓度在2.5~40.0 μg/mL范围内具良好线性关系(r=0.9995),检出限为0.8 mg/L(S/N=3),迁移时间和峰面积RSD分别为0.27%和1.8%,加标回收率90.3%～108.0%.用本法测定18名健康成人血浆中的牛磺酸,均值为15.8±3.2μg/mL.
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| 关 键 词： | 高效毛细管电泳法 牛磺酸 柱前衍生 血浆 |
Determination of taurine in human plasma by high performance capillary electrophoresis after pre-column derivatization with 9-fluorenylmethyl chloroformate |
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| XUE Yong,XUE Feng,SUN Cheng-jun. Determination of taurine in human plasma by high performance capillary electrophoresis after pre-column derivatization with 9-fluorenylmethyl chloroformate[J]. Chinese Journal of Analysis Laboratory, 2011, 30(6) |
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| Authors: | XUE Yong XUE Feng SUN Cheng-jun |
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| Affiliation: | XUE Yong,XUE Feng and SUN Cheng-jun(West China School of Public Health,Sichuan University,Chengdu 610041) |
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| Abstract: | |
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| Keywords: | HPCE Taurine Pre-column derivatization Plasma |
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