Hippurathydrolase,ein neues Enzym aus Mikroorganismen, 2. Mitt.: Enzymeigenschaften |
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Authors: | M. Röhr |
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Affiliation: | 1. Institut für Biochemische Technologie und Mikrobiologie der Technischen Hochschule Wien, Wien, ?sterreich
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Abstract: | Characteristic properties of the new enzymehippurate hydrolase are reported: - The Michaelis constant for hippurate hydrolysis is 5.5·10?4 mole/l at 5° and pH 7.5. The specific activity of the purified enzyme preparation is 7.5 μmole/minute · mg enzyme protein at 37° and pH 7.5. The pH optimum is 7.5.
- The effects of temperature on the reaction rate and on the stability of the enzyme were determined.
- The effects of various inhibitors (HgCl2, pCMB, EDTA, 8-hydroxyquinoline) and cations were studied. Participation of metal ions in the catalytic function ofhippurate hydrolase was not detectable.
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