| DNA cleavage promoted by diethylenetriaminepentaacetic acid （DTPA） derivatives |
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| 作者姓名： | Zhong Wei Yao Jun Xu Da Wei Zhang Hong Hui Lin Chuan Qin Xia |
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| 作者单位： | a College of Chemistry, Sichuan University, Chengdu 610064, China;b School of Life Science, Sichuan University, Chengdu 610064, China |
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| 基金项目： | supported by the National Natural Science Foundation of China(No.20301011) |
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| 摘 要： | DTPA and its two derivatives were synthesized and used as catalysts in DNA cleavage.Under physiological conditions,a series of experiments have been done.The effects of DNA cleavage with three ligands were studied under different concentrations, cleavage time,and pH values.The results strongly suggested that the plasmid DNA(pUC 19) can be cleaved efficiently by CITC-DTPA. For the cleavage reaction catalyzed by CITC-DTPA,FormⅠDNA could convert to FormⅡmostly,and the DNA-cleavage mechanism involved an oxid...
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| 关 键 词： | DNA裂解 衍生物 裂解催化剂 脱氧核糖核酸 DTPA 中国化学会 生理条件 实验工作 |
| 收稿时间： | 29 December 2008 |
DNA cleavage promoted by diethylenetriaminepentaacetic acid (DTPA) derivatives |
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| Zhong Wei Yao,Jun Xu,Da Wei Zhang,Hong Hui Lin,Chuan Qin Xia.DNA cleavage promoted by diethylenetriaminepentaacetic acid (DTPA) derivatives[J].Chinese Chemical Letters,2009,20(9):1107-1110. |
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| Authors: | Zhong Wei Yaoa Jun Xua Da Wei Zhangb Hong Hui Linb Chuan Qin Xia a College of Chemistry Sichuan University Chengdu China b School of Life Science China |
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| Institution: | a College of Chemistry, Sichuan University, Chengdu 610064, China;b School of Life Science, Sichuan University, Chengdu 610064, China |
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| Abstract: | DTPA and its two derivatives were synthesized and used as catalysts in DNA cleavage. Under physiological conditions, a seriesof experiments have been done. The effects of DNA cleavage with three ligands were studied under different concentrations,cleavage time, and pH values. The results strongly suggested that the plasmid DNA (pUC 19) can be cleaved efficiently by CITC- DTPA. For the cleavage reaction catalyzed by CITC-DTPA, Form I DNA could convert to Form Ⅱ mostly, and the DNA-cleavage mechanism involved an oxidative pathway. |
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| Keywords: | TPA Derivatives DNA cleavage Cleavage mechanism |
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