| 高灵敏选择性检测凝血酶的适体纳米银共振散射光谱探针 |
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| 引用本文： | 黄世稳,李纪顺,梁爱惠,蒋治良.高灵敏选择性检测凝血酶的适体纳米银共振散射光谱探针[J].化学学报,2011,69(2):183-189. |
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| 作者姓名： | 黄世稳 李纪顺 梁爱惠 蒋治良 |
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| 作者单位： | 1. 广西师范大学环境与资源学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,桂林,541004;右江民族医学院,百色,533000
2. 广西师范大学环境与资源学院珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,桂林,541004 |
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| 基金项目： | 国家自然科学基金(Nos.21075023,20965002,20865002); 广西自然科学基金(No.0991021Z); 广西环境工程与保护评价重点实验室基金资助项目 |
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| 摘 要： | 采用四氢硼钠制备了较稳定的纳米银,并用凝血酶(TB)适体修饰纳米银制各了识别凝血酶的适体纳米银探针.在pH 7.0的Tris-HCl缓冲溶液及KCl存在下,适体纳米银探针与凝血酶特异结合生成G-四分体和纳米银聚集体,导致体系在480 nm处的共振散射峰增强.随着凝血酶浓度的增大,生成的纳米银聚集体越多,共振散射强度线性...
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| 关 键 词： | 纳米银 适体 凝血酶 共振散射光谱法 |
Highly Sensitive and Selective Detection of Thrombin by Aptamer-modified Nanosilver Resonance Scattering Spectral Probe |
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| Huang Shiweng,Li Jishun,Liang Aihui,Jiang Zhiliang.Highly Sensitive and Selective Detection of Thrombin by Aptamer-modified Nanosilver Resonance Scattering Spectral Probe[J].Acta Chimica Sinica,2011,69(2):183-189. |
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| Authors: | Huang Shiweng Li Jishun Liang Aihui Jiang Zhiliang |
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| Institution: | Huang,Shiwenga,b Li,Jishuna Liang,Aihuia Jiang,Zhiliang,a(a Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,School of Environment and Resource Sciences,Guangxi Normal University,Guilin 541004)(b Youjiang Medical University for Nationalities,Baise 533000) |
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| Abstract: | Silver nanoparticles were prepared by NaBH4 reduction procedure.An aptamer-modified Ag nanoprobe(AgSH-ssDNA) for thrombin was prepared.In the media of pH 7.0 Tris-HCl and KCl,the AgSH-ssDNA specifically combined with thrombin to form G-quadruplex and Ag cluster,which resulted in the resonance scattering intensity at 480 nm(I480 nm) increasing.As the amount of thrombin increased,the amount of Ag clusters increased,and the I480 nm value increased.At the same time,Ag/Au nanoparticles of different Ag/Au molar r... |
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| Keywords: | nanosilver aptamer thrombin resonance scattering spectral assay |
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