Efficient N-terminal labeling of proteins by use of sortase |
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Authors: | Daniel J Williamson Martin A Fascione Michael E Webb W Bruce Turnbull |
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Affiliation: | School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT (UK) http://www.chem.leeds.ac.uk/MEW/ |
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Abstract: | "Sorting out" N-terminal labeling: The reversibility of transpeptidase reactions makes protein N-terminal labeling challenging. Depsipeptide substrates for sortase A release alcohol by-products, which are poor nucleophiles for the reverse reaction, during ligation. Proteins with an unhindered N-terminal glycine residue can be labeled efficiently with only a minimal excess of the labeling reagent. |
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Keywords: | depsipeptides enzymes peptides sortase transpeptidasen |
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