High-performance liquid chromatographic analysis of ampicillin and cloxacillin and its application to an intramammary veterinary preparation |
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| Authors: | D Thorburn Burns M O'Callaghan W Franklin Smyth and C J Ayling |
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| Institution: | (1) Department of Analytical Chemistry, The Queen's University of Belfast, BT9 5AG Belfast, Northern Ireland UK;(2) Department of Pharmacy, The Queen's University of Belfast, BT9 5AG Belfast, Northern Ireland UK;(3) Norbrook Laboratories, Station Works, BT35 6JP Newry, Northern Ireland UK;(4) Present address: Department of Applied Physical Sciences, University of Ulster, Coleraine, Co., BT52 1SA Londonderry, Northern Ireland UK |
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| Abstract: | Summary Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40–60°C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n=6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products. |
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