Analysis of tropane alkaloids with thermospray high-performance liquid chromatography-mass spectrometry |
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| Authors: | S Auriola A Martinsen K M Oksman-Caldentey T Naaranlahti |
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| Institution: | Department of Pharmaceutical Chemistry, University of Kuopio, Finland. |
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| Abstract: | A thermospray high-performance liquid chromatography-mass spectrometry method for analysis of hyoscyamine and scopolamine in plant cell culture samples is described. The alkaloids were separated on a polymeric reversed-phase column with an alkaline ammonium acetate buffer-acetonitrile eluent. Selected-ion recording of the protonated molecular ions was used for quantitation of the compounds. The compounds were fragmented by discharge-assisted ionization and elevated thermospray capillary temperatures or ion repeller potentials. |
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