填充条件对半制备柱性能影响的机理研究

对稀匀浆法填充半制备柱(Ф10 mm×100 mm)的过程进行研究,并用正交法对柱填充条件进行了优化,考察了压力、匀浆液组成、匀浆液体积对半制备柱填充效果的影响.由柱效和分离度定义式中的参数入手,讨论填充条件对柱性能的影响.通过计算总孔隙率ε_t、内部孔隙率ε_p、外部孔隙率ε_e、Van-Deemter曲线、吸附等温线等参数,发现填充压力对填充密度的作用最大,是最重要的影响因素;匀浆配比和匀浆体积共同影响其填充的均匀性.在压力11 MPa,流速275 mL/min,120 mL纯乙醇为匀浆液的条件下得到了最好的填充效果.

Study on Effects of Packing Conditions on the Performance of Semi-preparative Column

The semi-preparative columns(Ф10 mm×100 mm) were packed by homogenate slurry method.The packing conditions,such as pressure,packing solvent and homogenate volume were optimized by the orthogonal experiment.The influence of the packing conditions was discussed from the parameters of the column efficiency and the resolutions defining equation.The total porosity(ε_t),internal porosity(ε_p),external porosity(ε_e),Van-Deemter plot,adsorption isotherm were calculated.The result shows that pressure is the most important affecting factor for the density,and packing solvent and homogenate volume affect the homogeneity of the column.The best column in this experiment was obtained with 120 mL of ethanol as packing solvent under using pressure of 11 MPa and flow rate of 275 mL/min.