Liposomes from novel photolabile phospholipids: light-induced unloading of small molecules as monitored by PFG NMR |
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Authors: | Wan Yongqin Angleson Joseph K Kutateladze Andrei G |
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Affiliation: | Contribution from the Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA. |
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Abstract: | A molecular dithiane-based approach to synthesis of novel photolabile phospholipids is developed. These lipids are used in formulations with egg-POPC and cholesterol to prepare light-sensitive liposomes. Irradiation of such liposomes in PBS buffer (medium pressure mercury lamp, Pyrex filter, lambda > 300 nm) significantly increases the bilayer permeability and accelerates the release of entrapped small organic molecules by an order of magnitude. A simple assay, based on (1)H or (19)F PFG NMR measurements of diffusion coefficients, is developed to monitor light-induced unloading of the probe molecules. |
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