葡萄糖传感器聚氨酯扩散限制膜响应动力学的研究

对采用聚氨酯薄膜作为扩散限制膜的葡萄糖传感器的响应动力学进行了分析与计算。通过对葡萄糖氧化酶（GOD）的米氏常数Km值及衡量扩散控制作用的Y值的计算，从理论上说明了扩散限制膜的引入是提高葡萄糖传感器响应线性范围的一种有效方法。计算结果表明：GOD经壳聚糖固定化后米氏常数Km从9.6减小到7.2mmol/L；随着制膜液浓度及浸渍次数的增加，Y值增加，线性范围扩大，与实验结果是一致的。

Response Dynamics of Glucose Sensor Based on the Polyurethane Diffusion Limited Membranes

The response dynamics of glucose sensor based on polyurethane diffusion limited membranes was studied. By calculating the values of Km and Y, it was explained theoretically that the introduction of polyurethane diffusion limited membranes was an effective method for extending the linear range of glucose snesor. The results indicate that the K m value of immobilized glucose oxidase reduces from 9.6 mmol/L to 7.2 mmol/L, the values of Y increase with the increasing of polyurethane concentration and dipping times. The calculated results are identical with our experiments.