| 基质辅助激光解吸电离飞行时间质谱技术分析三七和海兔神经连索内的皂甙组分 |
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| 引用本文： | 冯丽剑,黄琳,卓慧钦,黄河清. 基质辅助激光解吸电离飞行时间质谱技术分析三七和海兔神经连索内的皂甙组分[J]. 分析化学, 2009, 37(12) |
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| 作者姓名： | 冯丽剑 黄琳 卓慧钦 黄河清 |
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| 作者单位： | 厦门大学生命科学学院生物化学与生物技术学系,厦门,361005;厦门大学生命科学学院生物化学与生物技术学系,厦门,361005;化学化工学院化学生物学系,厦门,361005;厦门大学生命科学学院生物化学与生物技术学系,厦门,361005;近海海洋环境科学国家重点实验室,厦门,361005;化学化工学院化学生物学系,厦门,361005 |
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| 基金项目： | "973"项目，国家自然科学基金 |
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| 摘 要： | 基质辅助激光解吸电离飞行时间(MALDI-TOF)质谱技术能高效解吸三七提取液(Panaxnotoginseng Extraction, PNE)中的混合皂甙分子为皂甙离子,并供质量分析器检测与分析.选用MALDI-TOF 质谱技术直接分析色谱纯皂甙样品的纯度,其检测灵敏度优于反相高效液相色谱法(RP-HPLC).优化提取中药三七(Panax notoginseng, PN)的混合皂甙, 选用MALDI-TOF质谱技术直接分析PNE中的皂甙种类和相对含量,发现PNE至少含有20种不同分子结构的皂甙组分,其中人参皂甙(Ginsenoside) Rg1和三七皂甙(Notoginsenoside)R1含量相对较高.选用薄板层析法(TLC)制备PNE中的R1皂甙.MALDI-TOF质谱技术研究蓝斑背肛海兔(Notarcus leachiicirrosus Stimpson, NLCS)神经连索内的超微量R1的组成与分布.建立PNE皂甙的指纹图谱,并适合于评价中药三七的品质和分析体内超微量皂甙的代谢过程与机理.
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| 关 键 词： | 三七 皂甙 基质辅助激光解吸电离飞行时间质谱 指纹图谱 |
Saponins Composition of Both Panax Notoginseng and Aplysia Neural Connective Analyzed with Matrix-assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry |
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| FENG Li-Jian,HUANG Lin,ZHUO Hui-Qin,HUANG He-Qing. Saponins Composition of Both Panax Notoginseng and Aplysia Neural Connective Analyzed with Matrix-assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2009, 37(12) |
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| Authors: | FENG Li-Jian HUANG Lin ZHUO Hui-Qin HUANG He-Qing |
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| Affiliation: | FENG Li-Jian1,HUANG Lin1,3,ZHUO Hui-Qin1,2,HUANG He-Qing1,3 (Department of Biochemistry and Biotechnology,School of Life Sciences1,State Key Laboratory of Marine Environmental Science,College of Oceanography and Environmental Science2,The Key Laboratory of Chemical Biology of Fujian Province,College of Chemistry & Chemical Engineering3,Xiamen University,Xiamen 361005) |
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| Abstract: | Mixed saponin molecules in the extraction of Panax notoginseng(PNE) can be effectively desorpted into the molecular ionization for measurement and analysis by mass analyzer from matrix assisted laser desorption ionization-time of flight-mass spectrometry(MALDI-TOF MS).The saponin samples with chromatography purity were directly analyzed by MALDI-TOF MS,which indicated that the sensitivity of the method was higher than that of RP-HPLC.A technology of MALDI-TOF MS was directly employed to analyze the saponin ... |
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| Keywords: | Panax notoginseng saponin matrix-assisted laser desorption ionization-time of flight-mass spectrometry fingerprinting maps identification |
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