Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins |
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| Authors: | Schmidt Frank Lueking Angelika Nordhoff Eckhard Gobom Johan Klose Joachim Seitz Harald Egelhofer Volker Eickhoff Holger Lehrach Hans Cahill Dolores J |
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| Affiliation: | Max Planck Institute for Molecular Genetics, Berlin, Germany. |
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| Abstract: | We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources. |
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