| 浙贝母4品种及5种贝母遗传多样性的RAPD分析 |
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| 引用本文： | 陆含,朱世华,周书军,宋乐民,张林苗. 浙贝母4品种及5种贝母遗传多样性的RAPD分析[J]. 宁波大学学报(理工版), 2009, 22(1): 44-47 |
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| 作者姓名： | 陆含 朱世华 周书军 宋乐民 张林苗 |
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| 作者单位： | 宁波大学生命科学与生物工程学院,浙江,宁波,315211;宁波市鄞州区农业技术服务站,浙江,宁波,315100;宁波市鄞州区贝母研究所,浙江,宁波,315161 |
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| 摘 要： | 通过RAPD技术对浙贝母的多子、宽叶、狭叶（浙贝1号）和岭下4个品种和贝母属的浙贝母、川贝母、皖贝母、东贝母、伊贝母5个种的遗传多样性进行了分析，并对预筛选得到的9种多态性丰富的引物进行了RAPD扩增，得到了57条清晰可辨的扩增片段，其中48条多态性谱带，占总谱带数的84．21％．研究结果表明：4个品种之间，多子贝母与其他3个品种间遗传分化较大；5个贝母种之间的相似性系数为0．2105～0．8492，其中东贝母与浙贝母亲缘关系最近，伊贝母与其他贝母亲缘关系最远；筛选到的分子标记对于浙贝母不同品种和贝母不同种的鉴别具有重要的意义．
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| 关 键 词： | 贝母 遗传多样性 RAPD |
Specific RAPD Screening of Breeds in Fritillaria Thunbergii and Genetic Diversity Analysis of Five Species of Fritillaria |
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| LU Han,ZHU Shi-hua,ZHOU Shu-jun,SONG Le-min,ZHANG Lin-miao. Specific RAPD Screening of Breeds in Fritillaria Thunbergii and Genetic Diversity Analysis of Five Species of Fritillaria[J]. Journal of Ningbo University(Natural Science and Engineering Edition), 2009, 22(1): 44-47 |
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| Authors: | LU Han ZHU Shi-hua ZHOU Shu-jun SONG Le-min ZHANG Lin-miao |
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| Affiliation: | 1.Faculty of Life Science and Biotechnology;Ningbo University;Ningbo 315211;China;2.Yinzhou District Agriculture Technique Service Station;Ningbo 315100;3.Yinzhou District Fritillaria Research Institute;Ningbo 315161;China |
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| Abstract: | Randomly amplified polymorphic DNA(RAPD) is used to study the genetic diversity of four F.thunbergii varieties including addition of seeds,broad-leaf,narrow leaves(the breed has been identified as countries on the 1st of Fritillaria thunbergii),lingxia and five fritillaria species including F.thunbergii,F.cirrhosa,F.anhuiensis,F.thunbergii var.chekiangensis,F.pallidiflora.By prescreening,nine polymorphic primers are selected for further RAPD amplification.The results clearly display 57 identified amplicons ... |
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| Keywords: | RAPD |
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