Preparative separation of rhein from Chinese traditional herb by repeated high-speed counter-current chromatography |
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Authors: | Wei Yun Zhang Tianyou Ito Yoichiro |
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Institution: | Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology Application, Beijing 100035, PR China. |
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Abstract: | High-speed counter-current chromatography (HSCCC) was repeatedly used for isolation and purification of rhein from Rheum officinale Baill (Dahuang) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:7:5:5, v/v), which had been selected by analytical (HSCCC). Using two preparative units of the HSCCC centrifuge, about a 500 mg amount of the crude extract was separated, yielding 6.7 mg of rhein at a high purity of over 97%. |
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