Genetic Engineering of Caulobacter crescentus for Removal of Cadmium from Water |
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Authors: | Jigar Patel Qiong Zhang R Michael L McKay Robert Vincent and Zhaohui Xu |
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Institution: | (1) Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403–0208, USA;(2) Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403–0208, USA;(3) Department of Geology, Bowling Green State University, Bowling Green, OH 43403–0208, USA; |
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Abstract: | Hexa-histidine (6His) peptide was inserted to a permissive site of the surface layer (S-layer) protein RsaA of Caulobacter crescentus. The recombinant strain JS4022/p723–6H, expressing RsaA-6His fusion protein was examined for its ability to sequester Cd(II)
from the bacterial growth medium. When mixed with 1 ppm CdCl2, JS4022/p723–6H removed 94.3 ∼ 99.9% of the Cd(II), whereas the control strain removed only 11.4 ∼ 37.0%, depending on experimental
conditions. The effective contact time of the cells and Cd(II) was as short as 15 min. When higher concentrations of CdCl2 were tested, JS4022/p723–6H consistently demonstrated enhanced binding capacity over the control strain. At 15 ppm of Cd(II),
each gram of JS4022/p723–6H dry cells retrieved 16.0 mg of Cd(II), comparing to 11.6 mg g−1 achieved by the control strain. This work provides a potential cost-effective solution toward bioremediation of heavy metals
from aqueous systems. |
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