壳聚糖包裹硅胶载体印迹牛血红蛋白的研究

用壳聚糖包裹的硅胶为载体, 利用壳聚糖表面的氨基与戊二醛结合, 在硅胶表面引入醛基, 固定模板蛋白(牛血红蛋白). 用溶胶-凝胶过程再次包裹固定蛋白质的载体, 洗去模板蛋白后, 得到具有选择性识别的牛血红蛋白分子印迹聚合物. 用红外光谱(IR)、扫描电子显微镜(SEM)和元素分析对聚合物进行了表征, 结果表明, 载体表面成功地接枝了分子印迹聚合物. 选择性吸附实验结果表明, 分子印迹聚合物具有良好的识别性能, 能实现水溶液中牛血红蛋白的富集.

Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin

The silica modified with chitosan was synthesized as a carrier material.The glutaraldehyde was coupled with amido group of chitosan,and template protein-bovine hemoglobin was immobilized by aldehyde group.Then,the carrier was coated by sol-gel,after template protein was removed;molecularly imprinted polymer was prepared for selectively recognizing bovine hemoglobin(BHb).The results of IR and scanning electron microscope(SEM) demonstrated the formation of a grafting polymer layer on the modified silica gel s...