| 微量量热法研究两种生物碱与DNA的嵌插作用 |
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| 引用本文： | 王保怀,张有民,杨铭,苗培,王夔. 微量量热法研究两种生物碱与DNA的嵌插作用[J]. 物理化学学报, 1994, 10(1): 82-86. DOI: 10.3866/PKU.WHXB19940119 |
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| 作者姓名： | 王保怀 张有民 杨铭 苗培 王夔 |
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| 作者单位： | Institute of Physical Chemistry, Peking University, Beijing 100871, Research Laboratory of Natural and Biomimetic Drugs, Beijing Medical University, Beijing 100083 |
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| 摘 要： |
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| 关 键 词： | 生物碱 抗癌 嵌插 焓变 热谱图 |
| 收稿时间： | 1992-05-16 |
| 修稿时间： | 1992-10-22 |
A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry |
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| Wang Bao-Huai,Zhang You-Min,Yang Ming,Miao Pei,Wang Kui. A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry[J]. Acta Physico-Chimica Sinica, 1994, 10(1): 82-86. DOI: 10.3866/PKU.WHXB19940119 |
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| Authors: | Wang Bao-Huai Zhang You-Min Yang Ming Miao Pei Wang Kui |
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| Affiliation: | Institute of Physical Chemistry, Peking University, Beijing 100871| Research Laboratory of Natural and Biomimetic Drugs, Beijing Medical University, Beijing 100083 |
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| Abstract: | The enthalpy changes for the binding of two alkaloids with the bioactivity to inhibit monoamine oxidase, harmaline(HL) and harmine(HM) to calf thymus DNA have been determined calorimetrically in a 0.01 mol·L~(-1) piperazine-N、N -bis(2-ethanesulfonic acid) buffer(pH=7.0) at 298.15 K by a MS-80 standard Calvet microcalorimeter. The results are △H_(298)(HL)=-10.7 kJ·mol~(-1), △H_(298)(HM)=-29.6 kJ·mol~(-1) respectively. According to the binding energy and thermokinetic curves from the experiments, the mechanism of intercalationm binding of two alkaloids to DNA has been discussed. |
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| Keywords: | Alkaloid Anticancer Intercalation Enthalpy change Thermogram |
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