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| 芦丁对罗丹明6G的荧光猝灭机理研究及其含量测定 | |
| 引用本文： | 刘晓慧,吕庆銮,王怀友.芦丁对罗丹明6G的荧光猝灭机理研究及其含量测定[J].化学分析计量,2011,20(4):39-41,47. |
| 作者姓名： | 刘晓慧 吕庆銮 王怀友 |
| 作者单位： | 1. 山东师范大学实验室管理处,济南,250014 2. 鲁南煤化工研究院,济宁,272000 3. 山东师范大学化学化工与材料科学学院,济南,250014 |
| 摘 要： | 根据Stem - Volmer方程和罗丹明6G吸收光谱的变化研究了芦丁对其荧光猝灭的类型及机理,结果表明,芦丁与罗丹明6G作用,形成一种稳定的复合物,而发生荧光静态猝灭,求得复合物的结合常数K=5.55×105L/mol,结合点数n=1.26.芦丁含量在0.92～137.8μg/mL范围内与F0/F成正比,检出限为0.... |
| 关 键 词： | 罗丹明6G 芦丁 荧光猝灭 Stem-Volmer方程 |
FLUORESCENCE QUENCHING MECHANISM OF RUTIN TO RHODAMINE 6G AND THE DETERMINATION OF RUTIN IN TABLETS |
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| Liu Xiaohui,LV Qingluan,Wang Huaiyou.FLUORESCENCE QUENCHING MECHANISM OF RUTIN TO RHODAMINE 6G AND THE DETERMINATION OF RUTIN IN TABLETS[J].Chemical Analysis And Meterage,2011,20(4):39-41,47. | |
| Authors: | Liu Xiaohui LV Qingluan Wang Huaiyou |
| Institution: | Liu Xiaohui(Laboratory and Equipment Management Office,Shandong Normal University,Jinan 250014,China) Lü Qingluan(Lunan Coal Chemical Industry Research Institute,Jining 272000,China) Wang Huaiyou (College of Chemistry,Chemical Engineering and Materials Science,China) |
| Abstract: | The quenching mechanism and type of Rutin to Rhodamine 6G was studied based on the Stern - Volmer plot and the change of UV - Vis absorption spectrum of Rhodamine 6G. The result showed that Rutin reacted w.ith Rhodamine 6G to form a stable complex so as to fluorescence static quenching process. The binding constant K（ 5.55 ×10^5 L/mol） and the number of binding sites n（1.26） were obtained. A linear relationship was obtained between Fo/F and the concentration of Rutin in the range of 0.92 - 137.8 μg/mL. The detection limit was 0.90μg/mL. A simple, rapid and precise method for determining Rutin was established based on the fluorescence quenching principle. According to the method, the result obtained for the determination of Rutin in tablet samples accorded with that determined by UV- Vis spectrophotometry. |
| Keywords: | Rhodamine 6G Rutin tablet fluorescence quenching Stern-Volmer equation |
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