G protein-coupled receptor mediated sensing of TMA |
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Authors: | Anke Suska Ana B. Ibáñez Pakorn Preechaburana Ingemar Lundström Anna Berghard |
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Affiliation: | 1. Division of Applied Physics, IFM, Linköping University, SE-58183 Linköping, Sweden;2. Department of Molecular Biology, Umeå University, SE-90187 Umeå, Sweden |
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Abstract: | A new approach for the detection of trimethylamine (TMA) using recombinant Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the olfactory epithelium, which conferred high sensitivity to TMA. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a convenient evaluation of TMA levels in fish tissue extracts. The developed gas measurement platform was able to detect TMA from 1 to 100 ppm within thirty-five minutes. |
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