A DNA hybridization assay using nanogold-streptavidin,electro-microchip and silver enhancement to analyze impedance signals |
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| Authors: | CH Yeh YH Chang HP Lin TC Chang YC Lin |
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| Institution: | 1. Department of Engineering Science, National Cheng Kung University, Taiwan;2. Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University, Taiwan;3. Department of Chemistry, National Cheng Kung University, Taiwan;4. Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Taiwan |
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| Abstract: | This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species. |
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