毛细管电泳微芯片在临床尿蛋白检测中的应用研究

用微芯片毛细管电泳法对临床患者尿蛋白进行了分离, 初步探讨了用于判断肾损伤的应用前景. 以pH 10.3, 75 mmol•L^－1的硼酸盐缓冲液作为芯片电泳缓冲体系, 利用蛋白质的紫外吸收特性, 在210 nm波段检测吸光度并进行信号收集和分析. 研究两种添加剂对提高尿蛋白分离效率的影响, 分析了肾病综合症、妊娠高血压症、风湿性心脏病和多发性骨髓瘤等患者尿样本, 并与美国Helena电泳系统分析结果对比, 得到了较一致的结果.

Capillary Electrophoresis Microchip Assay of Clinical Urinary Proteins

The separation and detection of urinary proteins have been conducted by microchip-based capillary electrophoresis (MCE), and application foreground of MCE in clinical diagnosis of various states of disease was discussed. Microchip-based capillary electrophoresis of urinary proteins could offer several advantages over standard urinary protein assay methods such as SDS-PAGE and conventional capillary electrophoresis, including the decrease of sample consumption and analysis time. The microchip used in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The characteristic absorbency near 210 nm of proteins was adopted under the condition: the electrophoretic buffer at 75 mmol•L^－1 borate with 0.5 mol•L^－1 lactate at pH 10.5. Urine samples from four clinical cases were selected as target for microchip-based urinary protein assays, and the results were consistent with those of commercial Helena Rapid Electrophoreses system.