藤三七中一个新黄烷醇和抗HIV活性成分

利用各种色谱(硅胶和凝胶)方法, 从藤三七[Boussingaultia gracilis Miers var. pseudobaselloides Bailey]的70%(体积分数)的乙醇提取物中分离得到2个黄烷醇类化合物(1, 2)和4个黄酮类化合物(3~6). 采用UV, IR, MS 和1D, 2D NMR方法, 分别鉴定出如下化合物: 7-羟基-5-甲氧基-8-甲基-6-甲酰基-3,4-黄烷二醇, 命名为藤三七醇A(1); 4,7-二羟基-5-甲氧基-8-甲基-6-甲酰基黄烷(2); 7-O-methylunonal(3); 5,7-二羟基-6, 8-二甲基-2-苯基-4H-1-苯并吡喃-4-酮(4); Desmosflavone(5)和Demethoxymatteucinol(6). 其中化合物1是一个新的黄烷二醇化合物, 化合物2~6为首次从该植物中分离得到. 抗HIV-1活性筛选结果表明: 化合物1, 2, 5, 6对HIV-1诱导合胞体的形成具有一定的抑制作用, 其半数有效浓度(EC50)分别为45.09, 48.73, 55.47 和 82.75 μmol/L, 治疗指数(TI)分别为1.41, 1.20, 7.15 和>8.51.

One New Flavanoid and Anti-HIV Active Constituents from Boussingaultia gracilis Miers var. pseudobaselloides Bailey

Boussingaultia gracilis Miers var. pseudobaselloides Bailey(Basellaceae) is a folk medicine used as an analgesic and supplements, only a few research was reported on the chemical constituents of this plant. This paper presented its chemical and anti-HIV active constituents. By column chromatography(Silica and Sephadex LH-20 gel) methods, two flavanols and four flavones were isolated from the 70%(volume fraction) ethanol extract. Based on extensive spectral analysis(UV, IR, MS and 1D, 2D NMR), compounds 1—6 were identified as bougracol A(1), 4,7-dihydroxy-5-methoxy-8-methyl-6-formyl-flavane(2), 7-O-methylunonal(3), 5,7-dihydroxy-6,8-dimethyl-2-phenyl-4H-1-benzopyran-4-one(4), desmosflavone(5) and demethoxymatteucinol(6). Among them, compound 1, a new flavan-diols, was deduced to be 7-hydroxy-5-methoxy-8-methyl-6-formyl-flavan-3,4-diols, and compounds 2—6 were firstly obtained from this plant. Anti-HIV assay suggests that compounds 1, 2, 5, 6 showed weak anti-HIV activities with EC50 values of 45.09, 48.73, 55.47 and 82.75 μmol/L, and TI values of 1.41, 1.20, 7.15 and >8.51, respectively.