基于可见吸收信号的乳酸脱氢酶光纤传感器

报道一种测定乳酸脱氢酶活力的基于可见吸收信号的光纤生物传感器，在该传感体系中，通过辅酶Ｉ的氧化还原对（ＮＡＤ＾＋／ＮＡＤＨ）将乳酸脱氢酶和心肌黄酶催化的两个脱氢过阳以耦合，第一个脱氢过程对分析对象进行了化学识别，第二个脱氢过程引起可见吸收信号的变化，该传感器对０～４００Ｕ／Ｌ的乳酸脱氢酶有线性响应关系，检测下限为４８ＵＬ，该传感器已用于人体血清中乳酸脱氢酶活力的测定。

A Fiber-optic Biosensor Based on Visible Absorption for the Dtermination of Lactate Dehydrogenase Activity

Lactate dehydrogenase activities can be determined by using a fiber-optic biosen-sor based on visible absorption. In this method two dehydrogenate procedures were catalyzedby lactate dehydrogenase and diaphorase respectively, and coenzyme I (NAD+/NADH) wasintroduced into the two enzyme reactions as an electron transfer. The first dehydrogenateprocedure is for the recognition of lactate dehydrogense and the second one will show achange of absorbance- The sensing element of the biosonsor consists of draphorase covalencebound on the tip of the fiber bundle. A linear response of the biosensor was obtained in therange of 0~400 U/L of lactate dehydrogenase with a detection limit of 48 U/L- This biosen-sor has been used to determine lactate dehydrogenase activities in human serum.