| L－天冬氨酸微生物电极的研制 |
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| 引用本文： | 王氢,邓家祺.L－天冬氨酸微生物电极的研制[J].高等学校化学学报,1994,15(7):974. |
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| 作者姓名： | 王氢 邓家祺 |
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| 作者单位： | 复旦大学化学系, 上海, 200433 |
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| 基金项目： | 国家自然科学基金，中国科学院长春应用化学研究所电分析化学开放实验室资助 |
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| 摘 要： | 本文以大肠杆菌为天冬氨酸脱氨反应的酶源,采用氨气敏电极为基础电极研制成L-天冬氨酸微生物电极。对细菌培养条件、固定方法及电极工作条件等进行了研究,测定L-天冬氨酸E～1gC曲线斜率为55mV,线性范围为6.0×10-4～8.0×10-3mol/L,寿命可达10天。
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| 关 键 词： | L－天冬氨酸 微生物电极 生物传感器 |
| 收稿时间： | 1993-07-05 |
A Microbial Electrode for L-Aspartate |
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| WANG Qing,DENG Jia-Qi.A Microbial Electrode for L-Aspartate[J].Chemical Research In Chinese Universities,1994,15(7):974. |
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| Authors: | WANG Qing DENG Jia-Qi |
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| Institution: | Department of Chemistry, Fudan University, Shanghai, 200433 |
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| Abstract: | An electrode for the determination of L-Aspartate was prepared.Escherichia coli was used as the biocatalyst and an ammonia gas electrode was used as the base electrode.The effects of the culture medium, immobilized methods of the bacteria and the response characteristics of the electrode were studied.The slope of the L-Aspartate response curve E～-IgCis 55 mV/decade, linear range is 6.0×10-4-8.0×10-3mol/Land life time is 10days. |
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| Keywords: | L-Aspartate Miocrobial electrode Biosensor |
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