Department of Analytical Chemistry, Faculty of Sciences, University of Cádiz, P.O. Box 40 E-11510-Puerto Real, Cádiz, Spain
Abstract:
A new analytical method has been developed for sample preconcentration and analysis of phenolic compounds in sherry wine using on-line solid-phase extraction(SPE)-HPLC-diode array detection. The samples of wine were injected and adsorbed onto polystyrene divinylbenzene cartridges; a robotic semiflexible system was used to automate the SPE stage. Chromatographic separation was carried out in a Symmetry C18 steel cartridge, with a two-step elution gradient. Peaks were identified by comparing their UV spectra with the library of spectra compiled by the authors.