Characterisation of polymers by size exclusion chromatography using multiple detection. Investigations on the determination of structural differences of hydroxyethyl starches |
| |
Authors: | K. Sommermeyer F. Cech E. Pfitzer K. Rößler |
| |
Affiliation: | (1) Pharmaceutical Division, Chemical Pharmaceutical Research and Development, Fresenius AG, Borkenberg 14, 6370 Oberursel/Tannus, Germany |
| |
Abstract: | Summary An aqueous size exclusion chromatography system is outlined using dual detection by a multi-angel laser light scattering photometer (MALLS) and a concentration detector (RI). This system makes possible the determination of the molecular weight distribution of water soluble polymers in conjunction with the radius of gyration. The differences in the radii of gyration at the same molecular weights of two hydroxyethyl starches with different molecular structure are presented qualitatively. The determination of the Mark-Houwink relation for these polymers leads to a qualitatively similar result. |
| |
Keywords: | Size exclusion chromatography Laser light scattering and RI detection Mark-Houwink constants Radius of gyration Hydroxyethyl starch |
本文献已被 SpringerLink 等数据库收录! |