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Optimizing ammonia pressurization/depressurization processing conditions to enhance enzymatic susceptibility of dwarf elephant grass
Authors:Ferrer  Alexis  Byers  Floyd M.  Sulbarán-de-Ferrer  Betzabé  Dale  Bruce E.  Aiello  Cateryna
Affiliation:(1) Food Laboratory, Department of Chemistry, Science Faculty, University of Zulia, Grano de Oro Módulo, Maracaibo, Venezuela;(2) Department of Animal Science, Texas A&M University, 101 Kleberg Center, 77843-2472 College Station, TX;(3) Department of Chemical Engineering, Michigan State University, A 202 Engineering Building, 48824-1226 East Lansing, MI;(4) Engineering Faculty, University of Zulia, Av. Goajira, Maracaibo, Venezuela
Abstract:An ammonia pressurization/depressurization process was investigated to evaluate the potential of producing reducing sugars from dwarf elephant grass, a warm-season forage. Moisture, temperature, and ammonia loading affected sugar yield (p<0.0001). At optimal conditions, ammonia processing solubilized 50.9% of the hemicellulose and raised the sugar yield (percentage of theoretical) from 18 to 83%. Glucose and xylose production were increased 3.2-and 8.2-fold, respectively. The mild processing conditions of the ammonia treatment (90–100°C, 5 min), the low enzyme loading (2 international filter paper units/g), and the short hydrolysis time (24 h), greatly enhance the potential of using for ages to produce sugars valuable for several applications.
Keywords:Ammonia  dwarfelephant grass  enzymatic hydrolysis  sugars
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