| 中压液相色谱纯化牛血红细胞超氧化物歧化酶 |
| |
| 引用本文： | 韩庆宏,汪振珊,徐晓莹,孙琴美,陈福来,张乃忠. 中压液相色谱纯化牛血红细胞超氧化物歧化酶[J]. 色谱, 1994, 12(1): 67-69 |
| |
| 作者姓名： | 韩庆宏 汪振珊 徐晓莹 孙琴美 陈福来 张乃忠 |
| |
| 作者单位： | 哈尔滨医科大学生化教研室 |
| |
| 摘 要： | A rapid and effective purification of superoxide dismutase from bovine erythrocytes by MPLC is repoted.After bovine erythrocytes were treated with chloroform-ethanol to remove hemoglobin,acetone was used toremove contaminant proteins.Then highly purified Cu-Zn SOD(the specific activity was 10 000～15 000U/mg pr.)was obtained by twice DEAE-Sepharose chromatography using different eluting procedures.SDS-PAGE of the Cu-Zn SOD purified as above revealed one band. The molecular weight was 1 5 000～17 000.
|
| 关 键 词： | 超氧化物歧化酶 红细胞. 中压液相色谱 |
Purification of Superoxide Dismutase from Bovine Blood by Medium Pressure Liquid Chromatography(MPLC) |
| |
| Han Qinghong,Wang Zhenshan,Xu xiaoying,Sun Qinmei,Chen Fulai , Zhang Naizhong. Purification of Superoxide Dismutase from Bovine Blood by Medium Pressure Liquid Chromatography(MPLC)[J]. Chinese journal of chromatography, 1994, 12(1): 67-69 |
| |
| Authors: | Han Qinghong Wang Zhenshan Xu xiaoying Sun Qinmei Chen Fulai & Zhang Naizhong |
| |
| Abstract: | |
| |
| Keywords: | medium pressure liquid chromatography superoxide dismutase erythrocyte |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
|
点击此处可从《色谱》下载全文 |
|
