Diverse macroporous spheres synthesized by multiple emulsion polymerization for protein analyses

Hydrophilic and hydrophobic spheres (1-2 mm) with macropores (50 μm) were synthesized by SDS stabilized O/W/O and W/O/W emulsion polymerization, respectively. The hydrophilic spheres were functionalized with trypsin for protein digestion and on-line integrated with the hydrophobic spheres for the subsequent sample clean-up and enrichment with almost no back pressure.