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A dual pH and temperature responsive polymeric fluorescent sensor and its imaging application in living cells
Authors:Yin Liyan  He Chunsheng  Huang Chusen  Zhu Weiping  Wang Xin  Xu Yufang  Qian Xuhong
Institution:State key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China.
Abstract:A polymeric fluorescent sensor PNME, consisting of A4 and N-isopropylacrylamide (NIPAM) units, was synthesized. PNME exhibited dual responses to pH and temperature, and could be used as an intracellular pH sensor for lysosomes imaging. Moreover, it also could sense different temperature change in living cells at 25 and 37 °C, respectively.
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