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Surface reconstitution of glucose oxidase onto a norbornylogous bridge self-assembled monolayer
Authors:Jingquan Liu  Michael N Paddon-Row  J Justin Gooding
Institution:School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
Abstract:An electrode construct was fabricated in which a self-assembled monolayer containing a novel norbornylogous bridge was covalently attached to flavin adenine dinucleotide (FAD), the redox active centre of several oxidase enzymes. The electrochemistry of the construct was investigated before and after the reconstitution of glucose oxidase around the surface bound FAD. Rapid rates of electron transfer were observed both before and after the reconstitution of biocatalytically active enzyme. However, no biocatalytic activity was observed under anaerobic conditions suggesting the a lack of enzyme turnover through direct electron transfer. It is proposed that a decrease in the electronic coupling between the redox active FAD and the electrode following reconstitution of the glucose oxidase – a probable consequence of the FAD being immersed in a protein environment – was responsible for the inability of the enzyme to be turned over under anaerobic conditions.
Keywords:Electron transfer  Glucose oxidase  Norbornylogous bridge  Self-assembled monolayers  Protein electrochemistry
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