Sensitive high-performance anion-exchange chromatographic determination of paeoniflorin and albiflorin by pulsed amperometric detection after solid-phase extraction |
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Authors: | Hee-Jung Sim Ji-Seon Jeong Ha-Jeong Kwon Yong-Moon Lee Seon-Pyo Hong |
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Institution: | 1. Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea;2. Department of Preventive and Social Dentistry & Institute of Oral Biology, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea;3. College of Pharmacy, CBITRC, Chungbuk National University, Gaesin-dong, Heung deok-gu, Chongju 361-763, South Korea |
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Abstract: | We developed a method to simultaneously determine paeoniflorin and albiflorin levels using high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPAEC-PAD). The main principle of our method includes solid-phase extraction step using Amberlite XAD-2 sorbent to remove sugars and to selectively determine glycosides by PAD. Under these conditions, the linear dynamic range was 0.01–100 μg/mL, and the albiflorin and paeoniflorin detection limits (S/N = 3) were 5 and 10 pg, respectively. The intra- and inter-day precisions (RSDs) were <5.07%, and the average recoveries from Paeoniae Radix and Si-ni-san ranged from 97.12 to 101.15%. Our method showed high selectivity, high sensitivity, and good repeatability for analyzing albiflorin and paeoniflorin in oriental medicinal preparation. |
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Keywords: | Anion-exchange chromatography PAD Solid-phase extraction Paeoniae Radix Paeoniflorin Albiflorin |
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