Separation and identification of neutral cereal lipids by normal phase high-performance liquid chromatography,using evaporative light-scattering and electrospray mass spectrometry for detection |
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| Authors: | João M Rocha Paavo J Kalo Velimatti Ollilainen F Xavier Malcata |
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| Institution: | 1. CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal;2. Department of Food and Environmental Sciences, P. O. Box 27 (Latokartanonkaari 11), FIN-00014 University of Helsinki, Finland;3. ISMAI – Instituto Superior da Maia, Avenida Carlos Oliveira Campos, P-4475-690 Avioso S. Pedro, Portugal;4. ITQP – Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, P-2781-901 Oeiras, Portugal |
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| Abstract: | A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3 μm-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour. |
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| Keywords: | Simple lipids Acylglycerols Free sterols Fatty acids Normal phase liquid chromatography Evaporative light-scattering detection Positive electrospray ionization tandem mass spectrometry |
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