Comprehensive two-dimensional liquid chromatography: Ion chromatography × reversed-phase liquid chromatography for separation of low-molar-mass organic acids |
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| Authors: | Stella S Brudin Robert A Shellie Paul R Haddad Peter J Schoenmakers |
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| Institution: | 1. Analytical Chemistry Group (HIMS), University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands;2. Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia;3. Analytical Development Product Chemistry, Syngenta, Jealott''s Hill International Research Centre, Bracknell, Berkshire RG42 6EY, UK |
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| Abstract: | In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt. |
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| Keywords: | Ion chromatography Reversed phase Organic acids Multidimensional liquid chromatography Orthogonal separation |
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