Qualitative drug analysis of hair extracts by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry |
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Authors: | Bill Guthery Tom Bassindale Alan Bassindale Colin T. Pillinger Geraint H. Morgan |
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Affiliation: | 1. Planetary and Space Sciences Research Institute, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK;2. Institute of Environmental Science and Research Ltd., Kenepuru Science Centre, 34 Kenepuru Drive, PO Box 50-348, Porirua, New Zealand;3. Department of Chemistry and Analytical Sciences, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK |
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Abstract: | A technique using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC × GC/TOFMS) is applied to a qualitative analysis of three sample extracts from hair suspected of containing various drug compounds. The samples were also subjected to a quantitative target analysis for codeine, morphine, 6-monoacetylmorphine (6-MAM), amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), methylenedioxymethylamphetamine (MDMA), methadone, and benzylpiperazine (BZP) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). GC × GC/TOFMS provided a non-specific procedure that identified various drugs, metabolites, and impurities not included in the target analysis. They included cocaine, diazepam, and methaqualone (quaalude). Comprehensive GC × GC separation was achieved using twin-stage cryo-modulation to focus eluant from a DB-5ms (5% phenyl) to a BPX50 (50% phenyl) GC column. The TOF mass spectrometer provided unit mass resolution in the mass range m/z 5–1000 and rapid spectral acquisition (≤500 spectra/s). Clean mass spectra of the individual components were obtained using mass spectral deconvolution software. The ‘unknown’ components were identified by comparison with mass spectra stored in a library database. |
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Keywords: | GC × GC/TOFMS Orthogonal separation Qualitative drug analysis Hair Mass spectral deconvolution Cryo-modulation |
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