A novel method for the determination of total 1,3-octanediols in apple juice via 1,3-dioxanes by solid-phase microextraction and high-speed gas chromatography |
| |
Authors: | Daniel Dí az Llorente,Pilar Arias Abrodo,Enrique Dapena de la Fuente,Juan José Mangas Alonso,Maria Dolores Gutié rrez Á lvarez,Domingo Blanco Gomis |
| |
Affiliation: | 1. Departamento de Química Física y Analítica, Universidad de Oviedo, C/ Julián Clavería 8, E-33006 Oviedo, Spain;2. Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Carretera de Oviedo s/n, E-33300 Villaviciosa, Spain |
| |
Abstract: | In this work, a novel, simple and fast method based on solid-phase microextraction (SPME) followed by high-speed gas chromatography (HSGC) was developed for the analysis of total 1,3-octanediols in apple juices by means of derivatization reaction to volatile 1,3-dioxanes. The derivatization reaction, SPME conditions, glycosidically bound fraction and 1,3-nonanediol as a surrogate standard were studied. The formation of 1,3-dioxanes from 1,3-diols was confirmed by GC–MS. The method was validated obtaining a regression coefficient (r2) of 0.9996, precisions between 0.3 and 9.8%, extraction recoveries in the range 94.7–112.2% and LOD of 2.9 μg l−1. Experimental design has been employed in the optimization of extraction factors and robustness assessment. The method was applied to the analysis of 21 Asturian apple varieties finding a double reciprocal relationship between the concentrations of saturated and unsaturated 1,3-octanediol. |
| |
Keywords: | High-speed gas chromatography Gas chromatography&ndash mass spectrometry Apple juice Solid-phase microextraction 1,3-Octanediols 1,3-Dioxanes Experimental design |
本文献已被 ScienceDirect 等数据库收录! |
|