Design and implementation of an array of micro-electrochemical detectors for two-dimensional liquid chromatography—Proof of principle |
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Authors: | Jude A. Abia Joel Putnam Khaled Mriziq Georges A. Guiochon |
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Affiliation: | 1. Department of Chemistry, The University of Tennessee, Knoxville, TN 37996-1600, USA;2. Division of Chemical Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA |
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Abstract: | Simultaneous two-dimensional liquid chromatography (2D-LC) is an implementation of two-dimensional liquid chromatography which has the potential to provide very fast, yet highly efficient separations. It is based on the use of time × space and space × space separation systems. The basic principle of this instrument has been validated long ago by the success of two-dimensional thin layer chromatography. The construction of a pressurized wide and flat column (100 mm × 100 mm × 1 mm) operated under an inlet pressure of up to 50 bar was described previously. However, to become a modern analytical method, simultaneous 2D-LC requires the development of detectors suitable for the monitoring of the composition of the eluent of this pressurized planar, wide column. An array of five equidistant micro-electrochemical sensors was built for this purpose and tested. Each sensor is a three-electrode system, with the working electrode being a 25 μm polished platinum micro-electrode. The auxiliary electrode is a thin platinum wire and the reference electrode an Ag/AgCl (3 M sat. KCl) electrode. In this first implementation, proof of principle is demonstrated, but the final instrument will require a much larger array. |
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Keywords: | Flat wide column Micro-electrochemical detectors Planar chromatography Two-dimensional chromatography |
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