Performance of columns packed with the new shell particles,Kinetex-C18 |
| |
| Authors: | Fabrice Gritti Irene Leonardis David Shock Paul Stevenson Andrew Shalliker Georges Guiochon |
| |
| Institution: | 1. Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA;2. Australian Centre for Research on Separation Science, University of Western Sydney, NSW, Australia |
| |
| Abstract: | The performance of the new Kinetex-C18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights for a number of low molecular weight compounds (e.g., anthracene and naphtho2,3-a]pyrene) were between 1.0 and 1.3, breaking the legendary record set 3 years ago by Halo-C18 packed columns. The liquid-solid mass transfer of proteins (e.g., insulin and lyzozyme) is exceptionally fast on Kinetex-C18 much faster than on the Halo-C18 column. The different contributions of dispersion and mass transfer resistances to the column efficiency were determined and discussed. The possible reasons for this extremely high column efficiency are discussed. |
| |
| Keywords: | Column packing technology Shell particles Van Deemter plot HETP Kinetex-C18 Halo-C18 Anthracene Naphtho[2 3-a]pyrene Bradykinin Insulin Lyzozyme Acetonitrile |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
