Determination of Organic Acids in Red Wine and Must on Only One RP-LC-Column Directly After Sample Dilution and Filtration |
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Authors: | Yan-Jun Zheng Yun-Tao Duan Yan-Fang Zhang Qiu-Hong Pan Jing-Ming Li and Wei-Dong Huang |
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Institution: | (1) College of Food Science and Nutritional Engineering, China Agricultural University, 100083 Beijing, China |
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Abstract: | A new method for simultaneous determination of organic acids in red wine and must by liquid chromatography was studied. The
determination of organic acids in wines can be achieved in less than 13 min, preceded only by a simple sample dilution and
filtration step. With this method, the chromatographic separation of eight organic acids and interfering peaks present in
red wine, required only one reversed phase column (Waters Atlantis dC18 column, 4.6 × 150 mm ID, 5 μm). As mobile phase, isocratic
acetonitrile–0.01 mol L−1 KH2PO4 at pH 2.7 5:95 (v/v) at a flow rate of 0.8 mL min−1 was used. Detection wavelength was set at 210 nm except for ascorbic acid which was detected at 243 nm. Application to red
wine and must confirmed good repeatability and showed a wide variation range for concentrations of organic acids. |
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